Optimization of the crystallizability of a single-chain antibody fragment
نویسندگان
چکیده
منابع مشابه
Construction of recombinant Pichia pastoris expressing single-chain antibody fragment against extracellular domain of EpCAM
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human epidermal growth factor receptor (her) family plays an important role in various types of cancers. as a result, antibodies against her and the mechanism of antigen-antibody binding action are under active investigation. we previously constructed a single-chain variable fragment (scfv) against her2, i.e. anti-her2 scfv, for expressing in the escherichia coli . in the present study, we repo...
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The pro-inflammatory cytokine, TNF-α, which plays a major role in the development and persistence of inflammatory diseases, is the basis for the use of anti-TNF-α therapies. The neutralization of TNF-α or blockage of its binding to the corresponding receptor has mainly served as a therapeutic strategy against some diseases. This study aimed to investigate the production of a humanized single ch...
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Human epidermal growth factor receptor (HER) family plays an important role in various types of cancers. As a result, antibodies against HER and the mechanism of antigen-antibody binding action are under active investigation. We previously constructed a single-chain variable fragment (ScFv) against HER2, i.e. anti-Her2 ScFv, for expressing in the Escherichia coli. In the present study, we repor...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology Communications
سال: 2014
ISSN: 2053-230X
DOI: 10.1107/s2053230x1402247x